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HiTrap Prepacked Columns

In 1991, six HiTrap columns were launched with the aim to simplify everyday protein purification. At a time when laboratory personnel spent hours, if not days, with the tricky task of packing small-scale columns, HiTrap prepacked columns became an immediate success. HiTrap offered researchers consistent quality, convenience, and significant time savings.

Over the years, the HiTrap family has grown to give researchers and process developers one of the widest choices of chromatography media. Today, there are over 170 different columns covering the most commonly used chromatography techniques—desalting, affinity, ion exchange, and hydrophobic interaction. The HiTrap family also includes a range of kits that streamline chromatography media screening and ease technology transfer from the lab bench to pilot scale to large-scale processes.

Much has happened since the first six columns launched in 1991, but their signature ‘red hat’ and quality remain. The HiTrap format is still as popular as ever, and if all the columns sold in 2013 were connected, they would build a chain almost 5 km (3 miles) long!

Easy to Connect and Scale-Up

HiTrap columns are available in 1-ml and 5-ml sizes and are made of biocompatible polypropylene. The columns can be directly coupled to ÄKTA chromatography systems without the need for connectors. They can also be used singly or connected in series for easy scale up together with a syringe, peristaltic pump, or chromatography system. To track column usage, the lot number and expiry date are printed on all HiTrap columns.

Application-Specific Column Packages

To simplify your way to success, we offer five application-specific HiTrap column packages. Each package consists of columns with high-performance media, supporting purification of tagged proteins, antibodies, or untagged (native) proteins.

His package for purification of histidine-tagged proteins

GST package for purification of GST-tagged proteins

Protein G package for purification of antibodies

Protein A package for purification of human antibodies

Untagged package for purification of untagged (native) proteins