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At various points during the generation and differentiation of ES cell lines, the cells are characterized to verify that they exhibit the fundamental properties of ES or the target cell.
Although scientists have not yet agreed on a standard battery of tests, they often include the growth and subculture of the cells over a period of many months, measurement of gene expression and cell surface markers, and characterization of cell morphology.
For adult stem cells, it must be demonstrated that a single adult-derived stem cell can generate a line of genetically identical cells that can then give rise to all the appropriate differentiated cell types of the tissue.However, much work remains to understand how to use these cells to their full potential.
Stem cell derived human neurones.
Huntington stem cell derived oligoendrocyte.
High-content analysis (HCA) is particularly valuable for stem cell analysis, providing high-throughput subcellular imaging and enabling comprehensive and fast analysis of pluripotency, differentiation, and phenotypic markers.
Combined with multiparameter supporting data on proliferation, cell morphology, and cell health, HCA brings a new dimension to stem cell research.
Our product portfolio for high-content analysis of stem cells includes:
In addition, our Biotrak Immunoassay Kits aid in the phenotypic and functional characterization of stem cells by measuring a range of intracellular analytes.
IN Cell Analyzer systems are designed to deliver the throughput and performance you need for both high-content analysis (HCA) and high-content screening (HCS) of fixed and live cells.
Detect low abundant secreted proteins or membrane surface markers using our range of immunoassays.
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